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Flinders
Proteomic Facility (FPF) and Laboratory
  
The Flinders Proteomic Facility (FPF) is a Flinders University Core laboratory which was established in 2002. It provides a broad range of standard and advanced research techniques for the analysis of proteins. The facility occupies laboratories 6E:101 and 6E:103 in the Flinders Medical Centre. The FPF supports research projects in a broad range of fields including neuroscience, biotechnology, cancer, ophthalmology, intracellular signalling and immunology. The advanced capabilities of the FPF attract researchers from major research institutions across Adelaide and interstate.
New Instrumentation and Software
- Thermo Orbitrap XL high resolution mass spectrometer
- Mascot Database Server
- Peaks de novo sequencing software
- Proteome Discoverer and SIEVE mass spectrometry software
- Polyscience recirculating chiller
- Bio Rad 1575 plate washer
*Some additional information is available in the FPF
Newsletter (Nov 09).
FPF instrumentation
- Dionex Ultimate 3000 nanoflow HPLC
- Thermo Orbitrap XL mass spectrometer
- Beckman Coulter ProteomeLab PF2D 2D HPLC
- Beckman Coulter Biomek 3000 robotic liquid handler
- GE Akta FPLC protein purification system
- GE Decyder 7.0 DIGE Analysis system
- 2 x GE IPGPhor3 isoelectric focusing units
- 2 x GE IPGPhor1 isoelectric focussing units
- 2 x GE Ettan 2D electrophoresis units
- GE Typhoon 9400 Fluorescence Imager
- 2 x Fuji LAS4000 imagers
- Beckman TLX Ultracentrifuge
- Misonix Sonicator 4000 with direct probe and cuphorn attachments
- UltraTurrax T10 homogenizer
- Millipore Snap I.D. blotting system
FPF supports these techniques
- 1D and 2D electrophoresis (mini-gel, midi-gel and Ettan format).
- 1D and 2D Western blots (ECL, ECF, cy dye).
- Sample preparation and cleanup
- Protein quantitation assay
- Western blotting
- Fluorescence / silver stain and imaging of 1D/2D gels
- DIGE (Differential Gel Electrophoresis)
- ELISA analysis
- Robotic preparation of samples for mass spectrometry.
- Protein sequencing by mass spectrometry
- Differential protein analysis by mass spectrometry
- De novo sequencing.
- Differential protein expression identification using ProteomeLab PF2D
- FPLC protein purification.
A cost recovery charge is levied to cover consumables and maintenance of equipment. Training is provided at no cost to academic institutions for projects performed in the FPF.
Investigators
Tim Chataway, BSc (Hons), PhD
Support Staff
Nusha Chegeni, Research Assistant
Students
Amy McCormack, PhD Student
Harsha Padmanabhan, Masters Biotech Student
Rutendo Marume, Masters Biotech Student
Contacts
Research Projects
Collaborative Research
Dr Jenny Hardingham and Georgia Arentz, Molecular Oncology Laboratory, Queen Elizabeth Hospital: Discovery of biomarkers for Colorectal Cancer using Proteomic methodology.
Michael Wiese (Royal Hobart Hospital), Prof Bob Heddle (Flinders University), Assoc Prof Simon Brown (University of Western Australia), Robert Milne (University of South Australia): Characterisation of ant venom proteins in Myrmecia pilosula (Jack Jumper) ant venom.
Prof Keryn Williams, Dr Alex Collela, Rhys Fogarty, Department of Ophthalmology, Flinders University: Heritable influences in an animal model of retinopathy of prematurity.
Assoc Prof Jamie Craig, Dr Shiwani Sharma, Dr Alex Hewitt, Department of Ophthalmology, Flinders University: Identification of the molecular basis of congenital cataract formation.
Prof Rose Ryall, Lauren Thurgood, Department of Surgery, Flinders University: Comparison of the complete intracrystalline protein profiles of urinary calcium oxalate monohydrate and dihydrate crystals.
Assoc Prof Dominic Thyagarajan, Ravi Raghupathi, Department of Neurology, Flinders Medical Centre: Characterisation of a novel mitochondrial mutation in the transcription termination region of the human mitochondrial genome.
Prof Simon Koblar, Michael Dkujic, Department of Medicine, University of Adelaide: Identification of blood biomarkers for patients experiencing trans ischaemic attacks.
Prof Tom Gordon, Dr Georgia Arentz, Department of Immunology, Allergy & Arthritis, Flinders University: Molecular characterisation of autoimmune antibodies in Sjögren's syndrome by mass spectrometry.
Dr Alan Wilson, Discipline of Anatomy & Histology, Flinders University: Characterisation of aquaporin-1 in rat brain.
Selected Recent Publications
Wiese MD, Davies NW, Chataway TK, Milne RW, Brown SG, Heddle RJ (2011) Stability of Myrmecia pilosula (Jack Jumper) Ant venom for use in immunotherapy. J Pharm Biomed Anal, 54:303-1
Wilson AJ, Carati CJ, Gannon BJ, Haberberger RV & Chataway
TK (2010) Aquaporin-1 in blood vessels of rat
circumventricular organs. Cell and Tissue Research, 340(1):
159-168
Thurgood LA, Wang T, Chataway TK, Ryall RL (2010) Comparison of the specific incorporation of intracrystalline proteins into urinary calcium oxalate monohydrate and dihydrate crystals. J Proteome Res, 9:4745-57
Sharma S, Chataway T, Burdon KP, Jonavicius L, Klebe S, Hewitt
AW, Mills RA, Craig JE (2009) Identification of
LOXL1 protein and Apolipoprotein E as components of surgically
isolated pseudoexfoliation material by direct mass spectrometry. Exp
Eye Res. 89, 479-485
Power J, Asad S, Chataway T, Chegini F, Manavis J, Temlett J,
Jensen P, Blumbergs P and Gai WP (2008) Peroxiredoxin
6 in human brain: molecular forms, cellular distribution and association
with Alzheimer’s disease pathology. Acta Neuropathology,
115, 611-622
Wiese MD, Milne RW, Davies NW, Chataway TK, Brown SG and Heddle
RJ (2008) Validation of a Procedure to Standardize
an Allergy Vaccine. Journal
of Pharmaceutical and Biomedical Analysis, 46, 58-65
O’Keefe L, Smibert P, Colella A, Chataway T, Saint
R and Richards R (2007) Know thy fly. Trends
in Genetics 23,
238-242
Wiese MD, Brown SG, Chataway TK, Davies
NW, Milne RW, Aulfrey SJ and Heddle RJ (2007)
Myrmecia pilosula (Jack Jumper) ant venom: identification
of allergens and revised. Allergy 62, 437-443
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